​Instructor: Nikki Wagar, CCSA, CLPE
​Course length: 24 hours

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Crime scenes involving bloodshed are often complex environments with vast opportunities to locate and preserve valuable evidence.

This three-day Detection and Enhancement of Bloodstain Evidence course is designed to provide crime scene investigators or laboratory personnel with the tools necessary to detect, enhance, and document blood evidence. 

Some crime scenes will present apparent blood evidence, while other crime scenes may be more complex and require search or enhancement techniques. Various chemical presumptive and enhancement techniques must be employed at crime scenes involving bloodshed to ensure thorough investigations are performed.

The Detection and Enhancement of Bloodstain Evidence course instruction will cover bloodstain detection, documentation, and preservation methods. Bloodstain search techniques will be employed to locate latent bloodstains within crime scenes and on items of potential evidentiary significance. Students will be introduced to various presumptive blood testing techniques as well as specialized chemical processing utilized to locate minute or cleaned up bloodstains. Friction ridge and footwear impressions deposited in blood are additional forms of evidence frequently located within bloody crime scenes. Sequential processing with multiple blood enhancement techniques will be employed to establish methods to develop impressions deposited in blood and capture comparison quality photographs to allow for future examinations. Practical exercises will be utilized throughout the course to enhance the learning experience.


Chemicals used in this course include, but are not limited to the following:

• Leucocrystal Violet (LCV)
• Amido Black
• Bluestar® Forensic
• Phenolphthalein
• Hungarian Red
• Ninhydrin
• Acid Yellow 7  

Nikki Wagar, CCSA, CLPE

Holding a master’s degree in Forensic Science, a bachelor’s degree in Physical Anthropology, and a certificate in Forensic Identification, Nikki Wagar is a Certified Latent Print Examiner and Certified Crime Scene Analyst through the International Association for Identification (IAI) and has been practicing both specialties for over 12 years.​

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